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cck 8  (Dojindo Labs)


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    Structured Review

    Dojindo Labs cck 8
    Cck 8, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 59922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cck-8+kit/Cell+Counting+Kit-8/pmc13085010-99-20-21
    Average 99 stars, based on 59922 article reviews
    cck 8 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Migration:


    Article Title: Monocyte-derived Kupffer cells dominate in the Kupffer cell pool during liver injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FBS Thermo Fisher Scientific Cat#A5669801 DAPI Vector Laboratories, Burlingame Cat#H1200 Critical commercial assays Mouse on Mouse Immunodetection Kit Vector Laboratories Cat#BMK-2202 CCK-8 kit Dojindo Molecular Technologies Cat#DH6615 PI/Annexin V kit BD Cat#555602D Foxp3/Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521 Deposited data RNA-seq data This paper CRA007105 Oligonucleotides See Table S1 for primers used in this study This paper N/A Software and algorithms LI-COR Odyssey Imaging System and Odyssey software LI-COR Biosciences Cat#926-32211 SPASS 23.0 IBM NA FlowJo 10.8.1 BD RRID:SCR_008520 Cytobank Beckman Coulter https://www.cytobank.cn/ Metascape Metascape https://metascape.org

    Article Title: Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction.
    Article Snippet: For cell proliferation experiments, cell viabilitywasevaluated using aCCK-8 kit (Dojindo) according to the manufacturer’s protocol.

    Article Title: Genome-wide CRISPR Screening Identifies the Pivotal Role of ANKRD42 in Colorectal Cancer Metastasis Through EMT Regulation
    Article Snippet: The assessment of cell viability was performed using a CCK-8 kit (#CK04, Dojindo Laboratories, Japan), following the manufacturer’s protocol.

    Article Title: Supporting Information
    Article Snippet: At the time of 1,3 and 5 days after inoculation, CCK-8 kit (Dojindo, Kumamoto, Japan) was applied to characterize cell proliferation.

    Article Title: Application of 3D bioprinting technology apply to assessing Dangguiniantongtang (DGNT) decoctions in arthritis.
    Article Snippet: To evaluate cell proliferation, the CCK-8 assay (Dojindo, Shanghai, China) was performed using the respective CCK-8 kit.

    Article Title: Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis
    Article Snippet: Cell counting kit-8 (CCK-8) assay The viability of the cells was determined by the CCK-8 kit (NU679, Dojindo Laboratories).

    Article Title: Clinical Value of MiR-636 in Postmenopausal Osteoporosis and Its Role in the Progression of Osteoporosis
    Article Snippet: Cell Proliferation, migration, and apoptosis CCK-8 kit was purchased from Dojindo (Japan).

    CCK-8 Assay:


    Article Title: Monocyte-derived Kupffer cells dominate in the Kupffer cell pool during liver injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FBS Thermo Fisher Scientific Cat#A5669801 DAPI Vector Laboratories, Burlingame Cat#H1200 Critical commercial assays Mouse on Mouse Immunodetection Kit Vector Laboratories Cat#BMK-2202 CCK-8 kit Dojindo Molecular Technologies Cat#DH6615 PI/Annexin V kit BD Cat#555602D Foxp3/Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521 Deposited data RNA-seq data This paper CRA007105 Oligonucleotides See Table S1 for primers used in this study This paper N/A Software and algorithms LI-COR Odyssey Imaging System and Odyssey software LI-COR Biosciences Cat#926-32211 SPASS 23.0 IBM NA FlowJo 10.8.1 BD RRID:SCR_008520 Cytobank Beckman Coulter https://www.cytobank.cn/ Metascape Metascape https://metascape.org

    Article Title: Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction.
    Article Snippet: For cell proliferation experiments, cell viabilitywasevaluated using aCCK-8 kit (Dojindo) according to the manufacturer’s protocol.

    Article Title: Genome-wide CRISPR Screening Identifies the Pivotal Role of ANKRD42 in Colorectal Cancer Metastasis Through EMT Regulation
    Article Snippet: The assessment of cell viability was performed using a CCK-8 kit (#CK04, Dojindo Laboratories, Japan), following the manufacturer’s protocol.

    Article Title: Supporting Information
    Article Snippet: At the time of 1,3 and 5 days after inoculation, CCK-8 kit (Dojindo, Kumamoto, Japan) was applied to characterize cell proliferation.

    Article Title: Application of 3D bioprinting technology apply to assessing Dangguiniantongtang (DGNT) decoctions in arthritis.
    Article Snippet: To evaluate cell proliferation, the CCK-8 assay (Dojindo, Shanghai, China) was performed using the respective CCK-8 kit.

    Article Title: Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis
    Article Snippet: Cell counting kit-8 (CCK-8) assay The viability of the cells was determined by the CCK-8 kit (NU679, Dojindo Laboratories).

    Article Title: Clinical Value of MiR-636 in Postmenopausal Osteoporosis and Its Role in the Progression of Osteoporosis
    Article Snippet: Cell Proliferation, migration, and apoptosis CCK-8 kit was purchased from Dojindo (Japan).

    Immunodetection:


    Article Title: Monocyte-derived Kupffer cells dominate in the Kupffer cell pool during liver injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FBS Thermo Fisher Scientific Cat#A5669801 DAPI Vector Laboratories, Burlingame Cat#H1200 Critical commercial assays Mouse on Mouse Immunodetection Kit Vector Laboratories Cat#BMK-2202 CCK-8 kit Dojindo Molecular Technologies Cat#DH6615 PI/Annexin V kit BD Cat#555602D Foxp3/Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521 Deposited data RNA-seq data This paper CRA007105 Oligonucleotides See Table S1 for primers used in this study This paper N/A Software and algorithms LI-COR Odyssey Imaging System and Odyssey software LI-COR Biosciences Cat#926-32211 SPASS 23.0 IBM NA FlowJo 10.8.1 BD RRID:SCR_008520 Cytobank Beckman Coulter https://www.cytobank.cn/ Metascape Metascape https://metascape.org

    Article Title: Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction.
    Article Snippet: For cell proliferation experiments, cell viabilitywasevaluated using aCCK-8 kit (Dojindo) according to the manufacturer’s protocol.

    Article Title: Genome-wide CRISPR Screening Identifies the Pivotal Role of ANKRD42 in Colorectal Cancer Metastasis Through EMT Regulation
    Article Snippet: The assessment of cell viability was performed using a CCK-8 kit (#CK04, Dojindo Laboratories, Japan), following the manufacturer’s protocol.

    Article Title: Supporting Information
    Article Snippet: At the time of 1,3 and 5 days after inoculation, CCK-8 kit (Dojindo, Kumamoto, Japan) was applied to characterize cell proliferation.

    Article Title: Application of 3D bioprinting technology apply to assessing Dangguiniantongtang (DGNT) decoctions in arthritis.
    Article Snippet: To evaluate cell proliferation, the CCK-8 assay (Dojindo, Shanghai, China) was performed using the respective CCK-8 kit.

    Article Title: Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis
    Article Snippet: Cell counting kit-8 (CCK-8) assay The viability of the cells was determined by the CCK-8 kit (NU679, Dojindo Laboratories).

    Article Title: Clinical Value of MiR-636 in Postmenopausal Osteoporosis and Its Role in the Progression of Osteoporosis
    Article Snippet: Cell Proliferation, migration, and apoptosis CCK-8 kit was purchased from Dojindo (Japan).

    RNA Sequencing:


    Article Title: Monocyte-derived Kupffer cells dominate in the Kupffer cell pool during liver injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FBS Thermo Fisher Scientific Cat#A5669801 DAPI Vector Laboratories, Burlingame Cat#H1200 Critical commercial assays Mouse on Mouse Immunodetection Kit Vector Laboratories Cat#BMK-2202 CCK-8 kit Dojindo Molecular Technologies Cat#DH6615 PI/Annexin V kit BD Cat#555602D Foxp3/Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521 Deposited data RNA-seq data This paper CRA007105 Oligonucleotides See Table S1 for primers used in this study This paper N/A Software and algorithms LI-COR Odyssey Imaging System and Odyssey software LI-COR Biosciences Cat#926-32211 SPASS 23.0 IBM NA FlowJo 10.8.1 BD RRID:SCR_008520 Cytobank Beckman Coulter https://www.cytobank.cn/ Metascape Metascape https://metascape.org

    Article Title: Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction.
    Article Snippet: For cell proliferation experiments, cell viabilitywasevaluated using aCCK-8 kit (Dojindo) according to the manufacturer’s protocol.

    Article Title: Genome-wide CRISPR Screening Identifies the Pivotal Role of ANKRD42 in Colorectal Cancer Metastasis Through EMT Regulation
    Article Snippet: The assessment of cell viability was performed using a CCK-8 kit (#CK04, Dojindo Laboratories, Japan), following the manufacturer’s protocol.

    Article Title: Supporting Information
    Article Snippet: At the time of 1,3 and 5 days after inoculation, CCK-8 kit (Dojindo, Kumamoto, Japan) was applied to characterize cell proliferation.

    Article Title: Application of 3D bioprinting technology apply to assessing Dangguiniantongtang (DGNT) decoctions in arthritis.
    Article Snippet: To evaluate cell proliferation, the CCK-8 assay (Dojindo, Shanghai, China) was performed using the respective CCK-8 kit.

    Article Title: Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis
    Article Snippet: Cell counting kit-8 (CCK-8) assay The viability of the cells was determined by the CCK-8 kit (NU679, Dojindo Laboratories).

    Article Title: Clinical Value of MiR-636 in Postmenopausal Osteoporosis and Its Role in the Progression of Osteoporosis
    Article Snippet: Cell Proliferation, migration, and apoptosis CCK-8 kit was purchased from Dojindo (Japan).

    Software:


    Article Title: Monocyte-derived Kupffer cells dominate in the Kupffer cell pool during liver injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FBS Thermo Fisher Scientific Cat#A5669801 DAPI Vector Laboratories, Burlingame Cat#H1200 Critical commercial assays Mouse on Mouse Immunodetection Kit Vector Laboratories Cat#BMK-2202 CCK-8 kit Dojindo Molecular Technologies Cat#DH6615 PI/Annexin V kit BD Cat#555602D Foxp3/Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521 Deposited data RNA-seq data This paper CRA007105 Oligonucleotides See Table S1 for primers used in this study This paper N/A Software and algorithms LI-COR Odyssey Imaging System and Odyssey software LI-COR Biosciences Cat#926-32211 SPASS 23.0 IBM NA FlowJo 10.8.1 BD RRID:SCR_008520 Cytobank Beckman Coulter https://www.cytobank.cn/ Metascape Metascape https://metascape.org

    Article Title: Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction.
    Article Snippet: For cell proliferation experiments, cell viabilitywasevaluated using aCCK-8 kit (Dojindo) according to the manufacturer’s protocol.

    Article Title: Genome-wide CRISPR Screening Identifies the Pivotal Role of ANKRD42 in Colorectal Cancer Metastasis Through EMT Regulation
    Article Snippet: The assessment of cell viability was performed using a CCK-8 kit (#CK04, Dojindo Laboratories, Japan), following the manufacturer’s protocol.

    Article Title: Supporting Information
    Article Snippet: At the time of 1,3 and 5 days after inoculation, CCK-8 kit (Dojindo, Kumamoto, Japan) was applied to characterize cell proliferation.

    Article Title: Application of 3D bioprinting technology apply to assessing Dangguiniantongtang (DGNT) decoctions in arthritis.
    Article Snippet: To evaluate cell proliferation, the CCK-8 assay (Dojindo, Shanghai, China) was performed using the respective CCK-8 kit.

    Article Title: Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis
    Article Snippet: Cell counting kit-8 (CCK-8) assay The viability of the cells was determined by the CCK-8 kit (NU679, Dojindo Laboratories).

    Article Title: Clinical Value of MiR-636 in Postmenopausal Osteoporosis and Its Role in the Progression of Osteoporosis
    Article Snippet: Cell Proliferation, migration, and apoptosis CCK-8 kit was purchased from Dojindo (Japan).

    Imaging:


    Article Title: Monocyte-derived Kupffer cells dominate in the Kupffer cell pool during liver injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FBS Thermo Fisher Scientific Cat#A5669801 DAPI Vector Laboratories, Burlingame Cat#H1200 Critical commercial assays Mouse on Mouse Immunodetection Kit Vector Laboratories Cat#BMK-2202 CCK-8 kit Dojindo Molecular Technologies Cat#DH6615 PI/Annexin V kit BD Cat#555602D Foxp3/Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521 Deposited data RNA-seq data This paper CRA007105 Oligonucleotides See Table S1 for primers used in this study This paper N/A Software and algorithms LI-COR Odyssey Imaging System and Odyssey software LI-COR Biosciences Cat#926-32211 SPASS 23.0 IBM NA FlowJo 10.8.1 BD RRID:SCR_008520 Cytobank Beckman Coulter https://www.cytobank.cn/ Metascape Metascape https://metascape.org

    Article Title: Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction.
    Article Snippet: For cell proliferation experiments, cell viabilitywasevaluated using aCCK-8 kit (Dojindo) according to the manufacturer’s protocol.

    Article Title: Genome-wide CRISPR Screening Identifies the Pivotal Role of ANKRD42 in Colorectal Cancer Metastasis Through EMT Regulation
    Article Snippet: The assessment of cell viability was performed using a CCK-8 kit (#CK04, Dojindo Laboratories, Japan), following the manufacturer’s protocol.

    Article Title: Supporting Information
    Article Snippet: At the time of 1,3 and 5 days after inoculation, CCK-8 kit (Dojindo, Kumamoto, Japan) was applied to characterize cell proliferation.

    Article Title: Application of 3D bioprinting technology apply to assessing Dangguiniantongtang (DGNT) decoctions in arthritis.
    Article Snippet: To evaluate cell proliferation, the CCK-8 assay (Dojindo, Shanghai, China) was performed using the respective CCK-8 kit.

    Article Title: Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis
    Article Snippet: Cell counting kit-8 (CCK-8) assay The viability of the cells was determined by the CCK-8 kit (NU679, Dojindo Laboratories).

    Article Title: Clinical Value of MiR-636 in Postmenopausal Osteoporosis and Its Role in the Progression of Osteoporosis
    Article Snippet: Cell Proliferation, migration, and apoptosis CCK-8 kit was purchased from Dojindo (Japan).



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    Image Search Results


    In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation

    doi: 10.1016/j.bioactmat.2026.04.003

    Figure Lengend Snippet: In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: On days 1, 3, and 5 of culture, the existing culture medium was substituted with 100 μL of CCK-8 working solution (Cell Counting Kit-8, Beyotime Biotech, China), prepared by mixing fresh α-MEM and CCK-8 reagent at a volume ratio of 10:1.

    Techniques: In Vitro, CCK-8 Assay, Cell Culture, Staining, Migration